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Immunoregulation of autocrine prolactin: Suppressing the expression of costimulatory molecules and cytokines in T lymphocytes by prolactin receptor knockdown
Authors:Dongming Xu  Ling Lin  Xiahong Lin  Zhenmin Lei
Affiliation:a Department of Rheumatology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China
b Department of Endocrinology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China
c Department of Heart Medicine, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China
d Department of Obstetrics, Gynecology and Women’s Health, University of Louisville School of Medicine, Health Sciences Center, Louisville, KY 40292, USA
Abstract:Ample evidence indicates that prolactin (PRL) secreted from the pituitary gland plays an important role in a variety of human immune responses. However, the immunoregulation of autocrine PRL in T lymphocytes is not fully understood. To evaluate the role of autocrine PRL in T lymphocyte activation, PRL receptor (PRLR) in Jurkat cells was silenced by lentivirus-mediated stable expression of PRLR shRNAi. Knockdown of PRLR resulted in a considerable reduction of phytohemagglutinin (PHA)-induced T cell proliferation. Moreover, the synthesis and secretion of CD137, CD154, IL-2 and IL-4 were significantly decreased, while the production of CD28, IFN-γ and IL-10 was not affected in PHA-primed PRLR-deficient cells. These results demonstrate the importance of autocrine regulation of the PRL signaling in T lymphocyte growth and activation, and support a mechanism by which autocrine PRL participates in the immunoregulation through selectively influencing the expression of certain critical costimulatory molecules and cytokines.
Keywords:Prolactin   Receptor   Lymphocytes   Cytokines   Immunoregulation   Bioassay   RNA interference
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