Combination of rapamycin and IL-2 do not affect antigen presentation ability of rat B cell and could promote Tregs proliferation and inhibitory activity |
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Authors: | Chuntao Zhang Jun Lu Li Feng Shengfu Li Youping Li |
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Institution: | a Key Laboratory of Transplant Engineering and Immunology of Health Ministry of China, West China Hospital, Sichuan University, Chengdu, 610041 Sichuan Province, People’s Republic of China b Hepato-bilio-pancreatic Surgery, West China Hospital, Sichuan University, Chengdu, 610041 Sichuan Province, People’s Republic of China c Chinese Cochrane Centre, Chinese Evidence-Based Medicine Centre, People’s Republic of China |
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Abstract: | Rapamycin (RPM), a powerful agent used clinically in transplant recipients, induces CD4+CD25+ regulatory T cells (Tregs) which play an important role in induction of immune tolerance. However, long-term use of RPM has negative side effects. In this report, we found that combination with the low dose RPM and high dose IL-2 did not affect antigen presentation of rat B cells to Tregs, and could efficiently promote Tregs proliferation and enhance their inhibitory activities in vitro. In addition, the combination of low dose RPM and high dose IL-2 enhanced mRNA expression of Foxp3, TGF-β1 and Pim-2 in Tregs but not in CD4+CD25− T effector cells (Teffs). The Tregs inhibitory activity is positively associated with mRNA expressions of TGF-β1 and Pim-2 while unrelated to the Foxp3 mRNA expression. Our present study offers one approach to expand functional Tregs in vitro, which maybe used for clinical immune tolerance induction. |
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Keywords: | Tregs CD4+CD25+ regulatory T cells RPM rapamycin Teffs CD4+CD25&minus T effector cells DC dendritic cells TGF-β1 transforming growth factor-β1 CAMK calcium/calmodulin-regulated kinase Pim proviral integration of MMLV LEW lewis rats BN brown Norway rats |
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