RELT induces cellular death in HEK 293 epithelial cells |
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Authors: | John K Cusick Andrea Mustian Mary E Reyland |
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Institution: | a University of Colorado Denver School of Dental Medicine, Department of Craniofacial Biology, 12801 East 17th Avenue, Aurora, CO 80045, USA b Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115-5819, USA |
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Abstract: | RELT is a recently identified Tumor Necrosis Factor Receptor that possess two homologues in humans named RELL1 and RELL2. We investigated whether RELT and its homologues could induce cellular death when transiently transfected into HEK 293 epithelial cells. Transfection of RELT family members into HEK 293 epithelial cells induced cell death characterized by rounding and lifting of cells accompanied by DNA fragmentation, characteristics that are consistent with the activation of an apoptotic pathway. Overexpression of RELT in COS-7 cells resulted in cell rounding and lifting without DNA fragmentation, suggesting that the effects of RELT signaling may vary among different cell types. In summary, we report that overexpression of RELT or its homologues RELL1 and RELL2 in HEK 293 epithelial cells results in cell death with morphological characteristics consistent with the activation of an apoptotic pathway. |
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Keywords: | RELT receptor expressed in lymphoid tissues RELL1 RELt like protein 1 RELL2 RELt like protein 2 TNF Tumor Necrosis Factor TNFR Tumor Necrosis Factor Receptor TRAF2 TNF receptor-associated factor 2 TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling |
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