Comparison of K,MgATPases in purified plasmalemma from wheat and oat. – Substrate specificities and effects of pH, temperature and inhibitors |
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Authors: | Marianne Sommarin Tomas Lundborg ers Kylin |
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Institution: | Dept of Plant Physiology, Univ. of Lund, P. O. B. 7007, S-220 07 Lund, Sweden;T. Lundborg, Dept of Crop Genetics and Breeding, The Swedish Univ. of Agricultural Sciences, Herman Ehles vag 2, S-268 00 Svalov, Sweden. |
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Abstract: | Plasmalemma from 8-day old oat ( Avena sativa L. cv. Brighton) and spring wheat ( Triticum aestivum L. cv. Drabant), grown in the dark at 18°C, was prepared from the 10000 g (10 min) – 30 000 g (60 min) root homogenate by two-phase separation in three steps with 6.5% (w/w) Dextran T 500 and 6.5% (w/w) polyethylene glycol 4 000. Biochemically and with respect to activation by Mg2+ as well as by (Mg2++ K+), the oat preparations clearly appeared as ATPase(s) in the pH range 5–8. They showed high specificity for ATP, temperature optima between 38 and 40°C, and were inhibited by vanadate, DCCD (dicyclohexylcarbodiimide) and SH-reagents, but not by oligomycin, ammonium molybdate or ouabain. In contrast, the preparations from wheat contained more than one type of MgATPase/ nucleotidase, as revealed by complex dependence on both pH and temperature as well as by comparatively low specificity towards nucleotides. However, no unspecific phosphatase was present, and the effect of K+ over and above that of Mg2+ was almost as specific as in oat by all criteria used. The data available from this and earlier investigations from our group would indicate that the complex reactions of preparations of wheat plasmalemma may not be due to contamination but, rather, expressions of the many biological functions that must be associated with the plasmalemma in vivo and which may be located in sub-units that are more firmly attached to wheat than to oat plasmalemma. |
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Keywords: | Avena sativa dicyclohexylcarbodiimide nucleoside phosphates oligomycin ouabain phase partitioning Triticum aestivum vanadate |
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