激素对枸杞体细胞胚发生及可溶性蛋白质含量和组分的影响

以宁夏枸杞无菌苗叶片为材料，对体细胞胚发生过程中激素作用、可溶性蛋白质变化及体细胞胚发生频率进行了研究。发现不同激素处理、可溶性蛋白质含量、组分和体细胞胚发生频率均有一定的差异，三者之间存在着相关性。结果如下：（１）ＭＳ＿１、ＭＳ＿２和ＭＳ＿３三种培养基上继代的愈伤组织难以诱导形成体细胞胚，为非胚性愈伤组织；而从ＭＳ＿１、ＭＳ＿２和ＭＳ＿３分别转到ＭＳ＿０和ＭＳ＿４培养基上的愈伤组织体细胞胚发生频率高，属于胚性愈伤组织。其中以ＭＳ＿３转至ＭＳ＿０后的体细胞胚发生频率最高。（２）可溶性蛋白质ＳＤＳ－ＰＡＧＥ分析表明：非胚性愈伤组织有特异性蛋白质６７ｋＤ，胚性愈伤组织有特异性蛋白质３５ｋＤ和４４ｋＤ；蛋白质３３ｋＤ和６７ｋＤ受２，４－Ｄ调控，蛋白质４９ｋＤ、５７ｋＤ受６ＢＡ调控，而蛋白质３７ｋＤ、５１ｋＤ受２，４－Ｄ和６ＢＡ协同调控。

EFFECT OF PLANT HORMONEAND THE CHANGES OF SOLUBLE PROTEINS ON SOMATIC EMBRYOGENESIS FROM LYCIUM BARBARUM

Bystudy on the effect of plant hormone,the changes of soluble proteins and the frequencyof somatic embryogenesis from Lycium barbarum,it was found that there may be certain relation-ship between them,The result showed that the frequency of somatic embryogenesis was the high-est when the callus was cultured with MS_0 medium after cultured with MS_3 medium. The SDS-PAGE patterns showed that non-embryonic callus predticed specific protein 67 kD,and embry-onic calltis produced specific proteins 35 kD and 44 kD.Inaddition,2,4-D regulated the genera-tion of proteins 33 kD and 67 kD,6 BA regulated the generation of proteins 49kD and 57kD,and proteins 37kD and 51kD were coordinately regulated by 2,4-Dand 6BA.