Purification of the toxic protein from Bacillus thuringiensis serotype 10 isolate demonstrating a preferential larvicidal activity to the mosquito |
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Authors: | K H Kim M Ohba K Aizawa |
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Institution: | Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan |
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Abstract: | The protein demonstrating larvicidal activity to the mosquito Aedes aegypti was purified from the alkali extract of the spore-parasporal inclusion complex of the isolate, 73-E-10-2, belonging to Bacillus thuringiensis serotype 10. By Sepharose CL-4B gel filtration and DEAE-cellulose column chromatography, a toxic protein was obtained, and its homogeneity was confirmed by Sephadex G-150 gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight of the toxic protein was 67,000, when estimated by SDS-PAGE. The LC50 of the toxic protein against 4-day-old larvae of A. aegypti was 16.8 μg/ml. There was no serological relationship between the toxic protein from the isolate 73-E-10-2 and that (Mr 67,000) from the type strain of B. thuringiensis subsp. israelensis. |
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Keywords: | toxic protein purification of mosquito larvae toxicity to To whom correspondence and reprint requests should be addressed |
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