光合细菌产辅酶Q10发酵条件的研究

利用均匀设计原理进行实验设计 ,对光合细菌R .capsulatusMT1131产辅酶Q10培养基配方及培养条件进行优化 ,结果当培养基中酵母膏质量浓度为 3 .13g·L- 1 ,硫酸铵 0 .8g·L- 1 ,Mg2 + 0 .6 4g·L- 1 ,Fe2 + 45 .2mg·L- 1 ,Mn2 + 18mg·L- 1 ,Co2 + 16mg·L- 1 ,培养基初始pH值为 7.0时 ,于 30℃ ,光照强度为 2 0 0 0Lx条件下培养 4天后 ,菌体中辅酶Q10质量浓度由 15 .2 13mg·L- 1提高至 2 0 .36 5mg·L- 1 ,产量提高约 33.87%。

Study on Conditions of Ubiquinone-10 Fermentation by Photosynthetic Bacteria

Uniform design was employed to optimize the conditions of ubiquinone-10 fermentation by photosynthetic bacteria. For maximal ubiquinone-10 production by R.capsulatus MT1131, an optimal culture medium of 3.13g·L -1 yeast extract, 0.8g·L -1(NH 4) 2SO 4, 0.64g·L -1 Mg 2+,45.2mg·L -1 Fe 2+,18mg·L -1 Mn 2+, 16mg·L -1 Co 2+ was required. When the strain grew in the optimal culture of initial pH 7.0, 30℃, 2000Lx for 4 days, the content of ubiquinone-10 in R.capsulatus MT1131 cells rose from 15.213mg·L -1 to 20.365mg·L -1 and increased by 33.87%.