Biomines-stimulated phosphorylation and (Na+, K+-ATPase in the gills of the chinese crab,Eriocheir sinensis |
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| Institution: | 1. School of Geography and Ocean Science, Nanjing University, 163 Xianlin Avenue, Qixia District, Nanjing, 210023, China;2. Carbon Neutralization and Territorial Optimization Laboratory, Ministry of Natural Resources, 163 Xianlin Avenue, Qixia District, Nanjing, 210023, China;3. Jiangsu Province Land Development and Consolidation Technology Engineering Center, Nanjing, 210023, China;4. Beijing Tsinghua Tongheng Urban Planning and Design Institute, Beijing, 100085, China;1. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China;2. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210093, China;3. Fujian Key Laboratory of Coastal Pollution Prevention and Control, Xiamen University, 361102, Xiamen, China;1. Behavioral Physiology and Sociobiology (Zoology II), Biozentrum, University of Würzburg, Am Hubland, Würzburg 97074, Germany;2. Brain Research Institute, University of Zürich, Winterthurerstrasse 190, Zürich CH-8057, Switzerland |
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| Abstract: | - 1.1. Subcellular distribution of (NA+, K+-ATPase and ouabain-insensitive ATPase (Mg2+-ATPase) are compared in branchial tissues of the euryhaline crab, Eriocheir sinensis, acclimated to fresh water.
- 2.2. Both the anterior and posterior gills contain cAMP-dependent protein kinase and endogenous protein substrate for phosphorylation.
- 3.3. Phosphorylation occurs in both “particulate” and “soluble” subcellular fractions but its stimulation by cAMP is restricted to the “soluble” fraction.
- 4.4. serotonin (5-HT) and dopamine receptors are present only in the “light particulate” fraction isolated from the posterior gills.
- 1.(a) Serotonin and dopamine have no effect on the phosphorylation observed in a subcellular fraction alone.
- 2.(b) Activation of the phosphorylation by serotonin and dopamine is found when the soluble fraction (source of cAMP-dependent protein kinase) is added to the fraction P3 from the posterior gills.
- 3.(c) No activation occurs with the fractions P3 as well as P1 or P2 (not shown) from anterior gills of fresh water crab.
- 4.(d) Cyproheptadine, a serotonin receptor antagonist, inhibits the 5-HT dependent increase in phosphorylation.
- 5.(e) The dopamine receptor antagonist, chlorpromazine, inhibits dopamine-stimulated phosphorylation.
- 6.5. Ouabain mimics the effect of cyproheptadine on the serotonin-stimulated phosphorylation found in the posterior gills.
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