Ion transport in crab gills: A new method using isolated half platelets of Eriocheir gills in an ussing-type chamber |
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| Institution: | 1. Department of Biology and Institute for Water Science, Wilfrid Laurier University, Waterloo, Ontario N2L 3C5, Canada;2. Department of Biosciences, University of Oslo, Oslo, Norway;3. Department of Biological Sciences, University of Alaska Anchorage, Anchorage, USA |
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| Abstract: | - 1.1. A half platelet preparation from Chinese crab (Eriocheir sinensis) gill is described which allows electrophysiological investigations of ion transport by gill epithelial monolayer when mounted in a modified Ussing chamber.
- 2.2. The resistance of these preparations equals half that of complete gill platelets (containing the gill epithelium and cuticle twice) indicating that cell damage during preparation of half platelets is negligible.
- 3.3. The transepithelial resistance (resistance of cuticle subtracted previously) was determined to be about 140 Ω cm2 when both sides are bathed with identical salines.
- 4.4. Similarities to the results obtained with perfused complete gills demonstrates the reliability of this preparation.
- 5.5. When identical salines are applied on both sides of the epithelium an outside positive transepithelial potential difference (PDte) up to 40 mV was measured.
- 6.6. The occurrence of such a high PDte under symmetric conditions and its sensitivity to CN− suggests the PDte to be generated by active transport processes.
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