Lipid agglutinins from coelomic fluid of the earthworm,Lumbricus terrestris |
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| Institution: | 1. Indian Institute of Technology Bombay, Mumbai, Maharashtra, 400076, India;2. Sierra Nevada Research Institute, University of California Merced, Merced, CA, 95343, USA;3. National University of Singapore, Queenstown, Singapore, 117568, Singapore;4. Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 237667, India;5. Deltaic Regional Centre, National Institute of Hydrology, Kakinada, Andhra Pradesh, 533003, India;1. Department of Polymer Science, University of Madras, Guindy Campus, Chennai 600025, India;2. Department of Zoology, University of Madras, Guindy Campus, Chennai 600025, India;1. Department of Metallurgical Technology Research, Central Iron and Steel Research Institute, Beijing 100081, China;2. Energy School, Xi''an University of Science and Technology, Xi''an, 710054, China;1. Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, M.S. Ramaiah University of Applied Sciences, New Bel Road, MSR Nagar, Bangalore, 560054, India;2. Department of Pharmacology, Faculty of Pharmacy, M.S. Ramaiah University of Applied Sciences, New Bel Road, MSR Nagar, Bangalore, 560054, India;3. Central Research Laboratory, Ramaiah Medical College, MSR Nagar, MSRIT Post, Bangalore, 560054, India;1. Biosciences Research, Department of Economic Development, Jobs, Transport and Resources, AgriBio, 5 Ring Road, Bundoora, Victoria, 3083, Australia;2. School of Applied Systems Biology, La Trobe University, Bundoora, Victoria, 3083, Australia;3. Imperial College London, Department of Surgery and Cancer, Sir Alexander Fleming Building, London, SW7 2AZ, UK;4. School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK |
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| Abstract: | - 1.1. Coelomic fluid of the earthworm, Lumbricus terrestris, contains lipid agglutinins that are primarily glycolipids. They are present in fluid from both immunized (induced) and unimmunized (naturally occurring) worms.
- 2.2. Lumbricus agglutinins partially purified by Folch extraction followed by silicic acid chromatography, were present in all fractions but in highest concentration in acetone and methanol fractions.
- 3.3. Immunodetection revealed agglutinin activity in acetone and methanol fractions, but not in the chloroform fraction.
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