Effects of membrane-perturbing drugs and noradrenalin on cAMP accumulation and red cell water content in carp (Cyprinus carpio) red cells |
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Institution: | 1. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China;2. College of Environment, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China |
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Abstract: | - 1.1. Carp red cells were treated with drugs that affect the cell membranes. The water content of the cells and the accumulation of cAMP in the cells were measured in normoxia and in hypoxia using non-stimulated and adrenergically stimulated cells.
- 2.2. WGA, DIDS + CCCP and A23187 increased the water content of nonstimulated normoxic cells.
- 3.3. In hypoxia ouabain and DIDS + CCCP increased the water content but cytochalasin B, NPM, DIDS, CCCP and A23187 + CA2+ abolished the hypoxia-induced swelling.
- 4.4. Any membrane perturbation induced some cAMP formation, Sophora and Anquilla lectins being most potent.
- 5.5. Also in adrenergically stimulated cells, membrane perturbation generally increased cAMP formation.
- 6.6. However, cAMP accumulation diminished in cells treated with cytochalasin B, CCCP and DIDS + CCCP.
- 7.7. The adrenergic swelling of carp red cells was reduced in normoxia by DIDS. NPM and CCCP increased the adrenergic swelling in normoxia to hypoxic level.
- 8.8. In hypoxia WGA and Anquilla lectin decreased the swelling.
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