Effects of high fat diets on hibernation and adipose tissue in Turkish hamsters |
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Authors: | Timothy J Bartness Rachel Milner Alain Geloen Paul Trayhurn |
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Institution: | (1) Department of Psychology, Georgia State University, Atlanta, Georgia, USA;(2) Department of Biology, Behavior and Neurobiology Program, Georgia State University, Atlanta, Georgia, USA;(3) Nutrition and Metabolism Research Group, Department of Foods and Nutrition, University of Alberta, 536 Newton Research Building, Edomonton, Alberta, Canada;(4) Nutrition and Metabolism Research Group, Department of Medicine, University of Alberta, 536 Newton Research Building, Edmonton, Alberta, Canada;(5) Division of Biochemical Sciences, Rowett Research Institute, Aberdeen, Scotland |
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Abstract: | Summary The effects of dietary fat saturation and fat content on hibernation and several properties of white and brown adipose tissue (WAT and BAT, respectively) were investigated in Turkish hamsters (Mesocricetus brandti). Male hamsters were housed in a long photoperiod (LD 16:8) at 23°C and fed one of three diets: (1) chow (6.5% fat per weight), (2) chow+13.5% vegetable oil (OIL, 20% fat per weight largely unsaturated fat]) and (3) chow+13.5% vegetable shortening SHORTENING, 20% fat per weight (largely saturated fat)]. Five weeks later body weights had stabilized and the animals were transferred to a short photoperiod (LD 8:16) at 3°C. At the peak of the hibernation season (17 weeks) the animals were sacrificed within 24 h of arousal. Chow-fed hamsters had the greatest percentage of animals hibernating and days found torpid compared with the two fat-fed groups, with no differences found between the latter two groups for these measures. There were no differences between hibernating (HIB) and nonhibernating (NON-HIB) hamsters across or within the diet groups for any of the BAT measures uncoupling protein content, mitochondrial mass, lipoprotein lipase (LPL) activity, and in vivo lipogenesis], nor were there significant effects of the diet on these measures. CHOW-and OIL-fed HIB hamsters showed decreases in body weight. All HIB groups had decreases in each carcass component, several fat pad weights, testes weight, and food intake. No consistent differences in WAT LPL activity or in vivo lipogenesis were found between HIB and NON-HIB hamsters. Feeding saturated high fat diets inhibits hibernation in some species; however, in the present experiment, feeding of both saturated and unsaturated fat-laden diets inhibited hibernation to a similar degree.Abbreviations
BAT
brown adipose tissue
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COA
cytochrome-c oxidase
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DS
dorsal subcutaneus
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DSWAT
dorsal subcutaneous white adipose tissue
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E
epididymal
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EWAT
epididymal white adipose tissue
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FFDM
fat-free dry mass
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HIB
hibernating
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I
interscapular
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IBAT
intercapsular brown adipose tissue
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IS
inguinal subeutaneus
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ISWAT
inguinal subcutaneous white adipose tissue
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LPL
lipoprotein lipase
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NON-HIB
non-hibernating
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R
retroperitoneal
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RWAT
retroperitoneal white adipose tissue
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SDS
sodium dodecyl sul
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UCP
uncoupling protein
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WAT
white adipose tissue |
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Keywords: | Hibernation High fat diet White adipose tissue Brown adipose tissue Thermogenesis Hamster Mesocricetus brandti Thermogenesis |
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