The tight junctions of rabbit choroid plexus and ciliary body epithelia. A comparative study using the double replica freeze-fracture technique |
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Authors: | M Hirsch D Verge C Bouchaud J Escaig |
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Institution: | 1. INSERM, Groupe U.86 (Pr. Dr. Y. Pouliquen), Clinique Ophtalmologique de PHôtel-Dieu France;2. Laboratoire de Cytologie, Université Pierre et Marie Curie France;3. Laboratoire de Technologie Appliquée à la Microscopie Electronique, CNRS, Paris, France |
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Abstract: | The spatial arrangement of tight junctions in choroid plexus and ciliary body rabbit epithelia has been determined by studying freeze-fracture complementary replicas. In the choroid plexus epithelium, the interruptions of the junctional P-face fibrils were measured to be 14% of their total length. In the ciliary body epithelium, where the fibrils were found to be more fragmented than in the choroid plexus, the P-face fibril interruptions accounted for 12 % of the total length of the zonulae occludentes sealing the non-pigmented cells and 30% in the focal linear tight junctions connecting the non-pigmented and pigmented cells at their apices. In both epithelia, the interruptions of the ridges are precisely complemented by particles or short bars of similar length found in the E-face furrows. Consequently, it is possible to conclude that the junctional fibrils are continuous in these two epithelia. For the zonulae occludentes, this continuity appears to be inconsistent with the ‘leaky’ properties of these epithelia shown by some physiological investigations. |
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Keywords: | Reprint requests to Mr Michel Hirsch Laboratoire de Cytologie Ultrastructurale Université Pierre et Marie Curie UER Broussais Hôtel-Dieu 15 rue de l'Ecole de Médecine 75270 Paris Cedex 06 France |
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