The nucleolus during epidermal development in an insect

The fifth stadium of Calpodes has two phases of epidermal cell development corresponding to preparation for intermoult and for moult syntheses. Both phases begin with a period of elevated RNA synthesis and the elaboration of a multilobed nucleolus. The apparent number of nucleoli changes from about two to eight and back to two again within the few hours of elevated RNA syntheses. The nucleolar changes are preceded by elevated litres of haemolymph ecdysteroid. During the two periods of activity, alveoli in the matrix of the nucleoli contain particles believed to be ribosomal precursors. The staining properties of these granules differ according to size in a way that suggests a developmental sequence. Mature granules are about 20 nm in diameter and do not stain with bismuth. They are found at the periphery of the nucleolus, in the nucleoplasm, at the approaches to and within the nucleopores. Perichromatin granules, believed to be m-RNA precursor packages, are up to 60 nm in diameter, do stain with bismuth and are found at the periphery of chromatin, in nucleoplasm and distorted at the approaches to the nuclear pores to fit within the central channel. During these periods of heightened activity the nuclear envelope contains microvesicles that may be free or attached to either nuclear or cytoplasmic surfaces. The structure is appropriate for the microvesicular transnuclear envelope movement of molecules such as the ecdysteroid believed to initiate the nuclear changes.