巨噬细胞吞噬过程中膜磷脂流动性和受体流动性的关系

本文用ＦＲＡＰ（ｆｌｕｏｒｅｓｃｅｎｃｅｒｅｃｏｖｅｒｙａｆｔｅｒｐｈｏｔｏｂｌｅａｃｈｉｎｇ）技术，测量了静息状态和刀豆素Ａ刺激不同时间后巨噬细胞膜磷脂、ＣｏｎＡ受体扩散系数和荧光恢复率的变化。结果显示ＣｏｎＡ刺激后膜磷脂和ＣｏｎＡ受体的扩散系数和荧光恢复率均较静息状态的巨噬细胞明显降低，磷脂流动性的变化与ＣｏｎＡ受体流动性的变化呈正相关。提示受体介导内吞导致的膜磷脂流动性的降低，可能是由于配体与细胞膜上受体结合形成配体－受体复合体，增加了受体的负荷，使受体的流动性降低，进而使膜磷脂的流动性降低。巨噬细胞内吞过程中膜磷脂和ＣｏｎＡ受体流动性的降低，可能还与ＣｏｎＡ刺激后巨噬细胞胞浆ｐＨ值有关。

RELATIONS BETWEEN MOBILITY OF MEMBRANE PHOSPHOLIPID AND CON A RECEPTORS DURING PHAGOCYTOSE IN MACROPHAGES

The diffusion coefficient and fluorescence recovery of macrophage membrane phospholipid and Con A receptors in resting condition and after stimulated by Concanavalin A (Con A) for different time were measured by FRAP (fluorescence recovery after photobleaching) technique. The results showed that the diffusion coefficient and fluorescence recovery of macrophages membrane phospholipid and Con A receptors evidently lowered after being stimulated by Con A than in resting conditions. The changes of mobility of phospholipid and that of Con A receptors were in positive relationship. We thought that the reduced mobility of Con A receptors was due to Con A receptors conjugated with Con A, added receptor's loading weight, thus leading to the reduction of Con A receptors and membrane phospholipid mobility. The reduction of mobility of Con A receptors and membrane phospholipid might be correlated to the acidification of cytosol induced by Con A.