Bioanalytical chiral chromatographic technique and docking studies for enantioselective separation of meclizine hydrochloride: Application to pharmacokinetic study in rabbits |
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| Authors: | Prachi Raikar Bannimath Gurupadayya Subhankar P Mandal Rishitha Narhari Sripuram Subramanyam Gunnam Srinivasu Surulivel Rajan Matcha Saikumar Sairam Koganti |
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| Institution: | 1. Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, India;2. Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, India;3. Daicel Chiral Technologies, Hyderabad, India;4. Department of Pharmacy Practice, Manipal College of Pharmaceutical Sciences, Manipal, India;5. Speciality Analytical Instrumentation Laboratory, Alphamed Formulation Pvt. Limited, Hyderabad, India |
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| Abstract: | Enantiomeric resolution and molecular docking studies of meclizine hydrochloride on polysaccharide-based chiral stationary phase comprising cellulose tris(4-methylbenzoate) chiral selector (150 × 4.6 mm, 3.0 μm) were presented. The mobile phase used was acetonitrile:10mM ammonium bicarbonate (95:05, v/v). The developed technique was used to perform the enantioselective assay of meclizine hydrochloride in its marketed formulation. The elution order of meclizine hydrochloride enantiomers was determined by docking studies. Target compound was extracted from rabbit plasma using protein precipitation technique, followed by development of bioanalytical chiral separation method using the same matrix. Application of the method to determine pharmacokinetic parameters of meclizine hydrochloride enantiomers was performed using Phoenix WinNonlin 8.1 software. The results demonstrated stereoselective disposition of meclizine hydrochloride enantiomers in rabbits. |
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| Keywords: | bioanalytical chiral docking meclizine hydrochloride pharmacokinetics |
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