The use of catechins in Chinese hamster ovary cell media for the improvement of monoclonal antibody yields and a reduction of acidic species |
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| Authors: | Luis Toronjo-Urquiza Adelina E. Acosta-Martin David C. James Tibor Nagy Robert J. Falconer |
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| Affiliation: | 1. Department of Chemical & Biological Engineering, ChELSI Institute, University of Sheffield, Sheffield, UK;2. biOMICS Facility, Faculty of Science Mass Spectrometry Centre, University of Sheffield, Sheffield, UK;3. Bioprocess Strategy and Development, Fujifilm Diosynth Biotechnologies, Stockton-on-Tees, UK;4. Department of Chemical Engineering and Advanced Materials, University of Adelaide, South Australia, Australia |
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| Abstract: | Catechin compounds have potential benefits for recombinant monoclonal antibody (Mab) production as chemical additives in cell culture media. In this study, four catechin compounds catechin (Cat), epicatechin (EC), gallocatechin-gallate (GCG), and epigallocatechin-gallate (EGCG) were added to cell culture media (at 50 μM) and their effects on the recombinant Chinese hamster ovary (CHO) cell culture, specific productivity, and Mab quality were assessed. The results indicate that the improvement of specific productivity was linked to cell growth inhibition. All catechins caused cell phase growth arrest by lowering the number of cells in the G1/G0 phase and increasing the cells in the S and G2/M phases. Late addition of the catechin resulted in a significantly higher final IgG concentration. Cat and EC caused an improvement in the final antibody titer of 1.5 ± 0.1 and 1.3 ± 0.1 fold, respectively. Catechins with a galloyl group (GCG and EGCG) arrested cell growth and reduced cell specific productivity at the concentrations tested. The Cat-treated IgG was found to have reduced acidic species with a corresponding increase in the main peak. |
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| Keywords: | antioxidant chemical chaperone flavan-3-ol flavanol flavonoids immunoglobulin |
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