Streptomyces thermocerradoensis I3 secretes a novel bifunctional xylanase/endoglucanase under solid-state fermentation |
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Authors: | Aline Rodrigues Gama Carolina Candida Q Brito-Cunha Ivan T N Campos Guilherme Rocha L de Souza Lilian Carla Carneiro Luiz Artur Mendes Bataus |
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Institution: | 1. Departament of Biochemistry and Molecular Biology, Federal University of Goiás, Goiânia, Brazil;2. Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Brazil |
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Abstract: | Lignocellulosic wastes can be potentially converted into several bioproducts such as glucose, xylo-oligosaccharides, and bioethanol. Certain processes, such as enzymatic hydrolysis, are generally needed to convert biomass into bioproducts. The present study investigated the production of xylanases and cellulases by Streptomyces thermocerradoensis I3 under solid-state fermentation (SSF), using wheat bran as a low-cost medium. The activities of xylanase and carboxymethyl cellulase (CMCase) were evaluated until 96 hr of incubation. The highest enzyme activity was observed after 72 hr of incubation. The crude enzyme extract was sequentially filtered, first using a 50 kDa filter, followed by a 30 kDa filter. Fraction 3 (F3) exhibited activities of both xylanase and CMCase. Xylanase and CMCase showed optimum activity at 70°C and pH 6.0 and 55°C and pH 6.0, respectively. The zymogram analysis showed a single activity band with a molecular mass of approximately 17 kDa. These findings provide strong evidence that the enzyme is a bifunctional xylanase/endoglucanase. This enzyme improved the saccharification of sugarcane bagasse by 1.76 times that of commercial cellulase. This enzyme has potential applications in various biotechnological procedures. |
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Keywords: | lignocellulosic wastes saccharification sugarcane bagasse wheat bran xylo-oligosaccharides |
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