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Simultaneous determination of ten aminoglycoside antibiotics in aquatic feeds by high-performance liquid chromatography quadrupole-orbitrap mass spectrometry with pass-through cleanup |
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| Authors: | Xiaoyi Lou Yunyu Tang Changling Fang Cong Kong Huijuan Yu Yongfu Shi Dongmei Huang Yaoguang Guo Dongxue Xiao |
| Affiliation: | 1. Laboratory of Quality Safety and Processing for Aquatic Product, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, Beijing, China;2. Laboratory of Quality Safety and Processing for Aquatic Product, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China;3. Research Centre of Resource Recycling Science and Engineering, School of Environmental and Materials Engineering, Shanghai Polytechnic University, Shanghai, China;4. Laboratory of Quality Safety and Processing for Aquatic Product, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China Department of Environmental Science & Engineering, Fudan University, Shanghai, China |
| Abstract: | A simple and sensitive method has been established based on pass-through cleanup and high-performance liquid chromatography quadrupole-orbitrap mass spectrometry (HPLC-Q/Orbitrap MS) for the simultaneous determination of ten aminoglycosides (AGs) in aquatic feeds. The extraction solution and cleanup procedure had been optimized, and good sensitivity, accuracy, and precision were obtained. The calibration curves of AGs were linearity (R2 > 0.99) in the range of 2.0 to 200 μg/L (or 5.0 to 500 μg/L). The limits of detection of AGs were between 10 and 25 μg/kg. The recoveries of AGs ranged from 74.9% to 94.3%, and the intraday and interday relative standard deviations were less than 15%. Finally, this method was successfully applied to determine ten AGs in 30 aquatic feed samples. It might be the first time to use pass-through cleanup approach combined with HPLC-Q/Orbitrap MS method for AGs determination in aquatic feed samples. |
| Keywords: | antibiotic residues exploration of detection method instrumental analysis modified solid phase extraction optimization |