Integration of ion exchange resin materials for a downstream-processing approach of an imine reductase-catalyzed reaction |
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Authors: | Lars-Erik Meyer Henrike Brundiek Jan von Langermann |
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Affiliation: | 1. Biocatalytic Synthesis Group, Institute of Chemistry, University of Rostock, Rostock, Germany;2. Enzymicals AG, Greifswald, Germany |
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Abstract: | In this study, an ion exchange resin-based downstream-processing concept for imine reductase (IRED)-catalyzed reactions was investigated. As a model reaction, 2-methylpyrroline was converted to its corresponding product (S)-2-methylpyrrolidine with >99% of conversion by the (S)-selective IRED from Paenibacillus elgii B69. Under optimized reaction conditions full conversion was achieved using a substrate concentration of 150 and 500 mmol/L of d -glucose. Seven commercially available cation- and anion-exchange resins were studied with respect to their ability to recover the product from the reaction solution. Without any pretreatment, cation-exchange resins Amberlite IR-120(H), IRN-150, Dowex Monosphere 650C, and Dowex Marathon MSC showed high recovery capacities (up to >90%). A 150-ml preparative scale reaction was performed yielding ~1 g hydrochloride salt product with >99% purity. Any further purification steps, for example, by column chromatography or recrystallization, were not required. |
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Keywords: | downstream-processing enzyme imine reductase ion exchange resin process development |
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