Efficacy of agar media for enumerating two Saccharomyces species in sucrose syrups |
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Authors: | L. R. Beuchat |
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Affiliation: | 1. Department of Food Science, University of Georgia, Agricultural Experiment Station, 30212, Experiment, GA, USA
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Abstract: | Experiments were carried out to determine the suitability of acidified and antibiotic-supplemented agars for supporting colony formation by cells ofSaccharomyces cerevisiae andSaccharomyces rouxii. Yeasts had been suspended in sucrose syrups buffered at pH 3.0, 5.0 and 7.0, and stored at 4 and 21°C for periods of time ranging to 10 weeks. TheSaccharomyces species were recovered in equal numbers, regardless of the type of enumeration agar. Sucrose, at concentrations up to 60%, protected cells against inactivation during storage, and survival was greater at 21° than at 4°C. |
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