A rapid and sensitive screening kit for the detection of anti-Toxocara larval ES antibodies |
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Authors: | Nobuaki Akao Albert E. Chu Setsuko Tsukidate Koichiro Fujita |
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Affiliation: | aDepartment of Medical Zoology, Faculty of Medicine, Tokyo Medicaland Dental University, 1-5-45 Yushima, Bunkyo-ku,Tokyo 113, Japan;bE.Y. Laboratories Inc., San Mateo, CA, USA |
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Abstract: | We developed a new screening kit for the detection of anti-Toxocara larval excretory-secretory antibodies. The test can be performed within 3 min for one sample and does not require a high-priced supplemental instrument. Moreover, results are easily and directly observed. Using this test kit, 22 sera taken from healthy subjects were negative for anti-Toxocara larval excretory-secretory antibodies at the serum dilution of 1:20. Of 14 proven cases of parasitic infections other than toxocariasis, one (gnathostomiasis) showed a positive result, but the others were negative. In serologically diagnosed toxocariasis, the test kit showed good correlation with ELISA, immunoblot and double gel diffusion tests. We designated this test kit as ToxocaraCHEK. |
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Keywords: | Toxocariasis Toxocara canis Serodiagnosis Vitreous fluid Rapid diagnosis |
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