Use of superoxide dismutase and catalase producing lactic acid bacteria in TNBS induced Crohn's disease in mice |
| |
Authors: | LeBlanc Jean Guy del Carmen Silvina Miyoshi Anderson Azevedo Vasco Sesma Fernando Langella Philippe Bermúdez-Humarán Luis G Watterlot Laurie Perdigon Gabriela de Moreno de LeBlanc Alejandra |
| |
Affiliation: | a Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, San Miguel de Tucumán, Tucumán T4000ILC, Argentina b Instituto de Microbiología, Universidade Federal de Minas Gerais (ICB/UFMG), Belo Horizonte, Brazil c UMR 1319 MICALIS-Microbiologie de l’Alimentation au Service de la Santé humaine, INRA-Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France d Cátedra de Inmunología, Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Argentina |
| |
Abstract: | Reactive oxygen species are involved in various aspects of intestinal inflammation and tumor development. Decreasing their levels using antioxidant enzymes, such as catalase (CAT) or superoxide dismutase (SOD) could therefore be useful in the prevention of certain diseases. Lactic acid bacteria (LAB) are ideal candidates to deliver these enzymes in the gut. In this study, the anti-inflammatory effects of CAT or SOD producing LAB were evaluated using a trinitrobenzenesulfonic acid (TNBS) induced Crohn's disease murine model. Engineered Lactobacillus casei BL23 strains producing either CAT or SOD, or the native strain were given to mice before and after intrarectal administration of TNBS. Animal survival, live weight, intestinal morphology and histology, enzymatic activities, microbial translocation to the liver and cytokines released in the intestinal fluid were evaluated. The mice that received CAT or SOD-producing LAB showed a faster recovery of initial weight loss, increased enzymatic activities in the gut and lesser extent of intestinal inflammation compared to animals that received the wild-type strain or those that did not receive bacterial supplementation. Our findings suggest that genetically engineered LAB that produce antioxidant enzymes could be used to prevent or decrease the severity of certain intestinal pathologies. |
| |
Keywords: | Lactic acid bacteria Superoxide dismutase Intestinal inflammation |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|