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Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin |
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| Authors: | Wang Zhenyu Li Jennifer Cheong Samantha Bhaskar Ujjwal Akihiro Onishi Zhang Fuming Dordick Jonathan S Linhardt Robert J |
| Affiliation: | a Department of Biology, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA b Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA c Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA d Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA e Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA |
| Abstract: | The chemical step in the chemoenzymatic synthesis of bioengineered heparin has been examined and optimized statistically using a response surface methodology. A four factor, two level full factorial design experiment and a three factor Box-Behnken design were carried out. The goal was to establish a method to prepare N-sulfo, N-acetyl heparosan of the desired N-acetyl content, number average molecular weight, and in maximum yield by controlling the reactant concentrations, reaction time and reaction temperature. The response surface models obtained were used to predict the reaction conditions required to optimally prepare N-sulfo, N-acetyl heparosan from Escherichia coli generated heparosan starting material of different molecular weights. |
| Keywords: | Bioengineered Heparin Heparosan N-deacetylation Factorial design |
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