Thermal treatment enhances the stability and biological activity of a truncated tilapia somatotropin contained in Pichia pastoris culture supernatant |
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Authors: | Jannel AcostaOdalys Ruiz Yamila CarpioReynold Morales Julio C AguilaJorge Valdés Eduardo MartínezMario P Estrada |
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Institution: | a Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana 10600, Cuba b Development Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana 10600, Cuba |
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Abstract: | The importance of somatotropin as a growth promoting agent and immune-stimulator has long been recognized and its potential application in the fish farming industry has been an active research area. In the work reported here, we sought to improve the stability of a previously obtained truncated somatotropin by applying a 60 °C heat shock to the culture supernatant containing this molecule, and then compared its effects with and without heat shock on larval growth and immune functions. We observed that the treatment with heat shock at 60 °C enhanced protein stability, growth and innate immune functions in tilapia larvae. |
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Keywords: | Fish Growth hormone Heat shock Protein stability Biological activity Pichia pastoris |
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