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High-quality genome sequence of Pichia pastoris CBS7435 |
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| Authors: | Küberl Andreas Schneider Jessica Thallinger Gerhard G Anderl Ingund Wibberg Daniel Hajek Tanja Jaenicke Sebastian Brinkrolf Karina Goesmann Alexander Szczepanowski Rafael Pühler Alfred Schwab Helmut Glieder Anton Pichler Harald |
| Institution: | a Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, A-8010 Graz, Austria b Austrian Centre of Industrial Biotechnology (ACIB), Petersgasse 14, A-8010 Graz, Austria c Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstraße 27, D-33615 Bielefeld, Germany d Bioinformatics Resource Facility, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstraße 25, D-33615 Bielefeld, Germany e Institute for Genomics and Bioinformatics, Graz University of Technology, Petersgasse 14, A-8010 Graz, Austria |
| Abstract: | The methylotrophic yeast Pichia pastoris (Komagataella phaffii) CBS7435 is the parental strain of commonly used P. pastoris recombinant protein production hosts making it well suited for improving the understanding of associated genomic features. Here, we present a 9.35 Mbp high-quality genome sequence of P. pastoris CBS7435 established by a combination of 454 and Illumina sequencing. An automatic annotation of the genome sequence yielded 5007 protein-coding genes, 124 tRNAs and 29 rRNAs. Moreover, we report the complete DNA sequence of the first mitochondrial genome of a methylotrophic yeast. Fifteen genes encoding proteins, 2 rRNA and 25 tRNA loci were identified on the 35.7 kbp circular, mitochondrial DNA. Furthermore, the architecture of the putative alpha mating factor protein of P. pastoris CBS7435 turned out to be more complex than the corresponding protein of Saccharomyces cerevisiae. |
| Keywords: | Pichia pastoris Genome sequence Genome annotation Mitochondrial genome Alpha mating factor |
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