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Aequorin-expressing yeast emits light under electric control |
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| Authors: | Vilanova Cristina Hueso Angeles Palanca Carles Marco Guillem Pitarch Miguel Otero Eduardo Crespo Juny Szablowski Jerzy Rivera Sara Domínguez-Escribà Laura Navarro Emilio Montagud Arnau de Córdoba Pedro Fernández González Asier Ariño Joaquín Moya Andrés Urchueguía Javier Porcar Manuel |
| Institution: | a Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, Postal Code 22085, 46071 València, Spain b Interdisciplinary Modeling Group, Intertech, Instituto Universitario de Matemática Pura y Aplicada, IUMPA, Departamento de Matemática Aplicada, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 València, Spain c Departamento de Lenguajes y Ciencias de la Computación, Campus de Teatrinos, Universidad de Málaga, 29071 Málaga, Spain d Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola 08193, Spain e Fundació General de la Universitat de València, Spain |
| Abstract: | In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5 V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control. |
| Keywords: | Aequorin Bioluminescence Yeast electro-stimulation Synthetic biology Bioelectronics |
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