Antibiotic Susceptibility Testing of Anaerobes: Comparison of Three Methods, Including an Automated Micro-method, and Evaluation of the Effect of Different Media |
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| Authors: | S P BORRIELLO SANDRA HEATON B S DRASAR |
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| Institution: | Bacterial Metabolism Research Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW9 5HT;Department of Microbiology, School of Hygiene and Tropical Medicine, Keppel Street &Gower Street, London WC1 7HT |
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| Abstract: | Disc-diffusion, agar-dilution and microdilution methods were compared for testing the antibiotic sensitivity of anaerobic bacteria. Eight antibiotics and 31 test organisms were used. There was a poor correlation between MIC values obtained by the agar-dilution and microdilution procedures. The test organisms showed an apparently increased sensitivity to the antibiotics when tested by the agar-dilution, as compared with the microdilution, procedures. Columbia Blood Agar and Thioglycollate Haemin agar, were evaluated for the disc-diffusion and agardilution procedures. There was a poor correlation between the MIC values and inhibition zone diameters for the two media, with lower MIC values and larger zones on Thioglycollate Haemin agar. |
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