Metabolism of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene by Alcaligenes denitrificans |
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Authors: | Rajiv Ahuja Niranjan Awasthi Natesan Manickam Ashwani Kumar |
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Affiliation: | (1) Environmental Biotechnology Section, Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow, 226 001, India |
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Abstract: | Metabolism of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE), a persistent metabolite of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT), by an Alcaligenes denitrificans was optimal under `non-shaking' conditions, was accelerated by adding 1 g glucose l–1, and inhibited by 1 g sodium acetate l–1 or 1 g sodium succinate l–1. Addition of biphenyl, in the vapor form, to the reaction mixture did not enhance DDE metabolism. During the reaction, accumulation of conventional metabolites, 1-chloro-2,2-bis(4-chlorophenyl)ethylene (DDMU) and 4-chlorobenzoate, was not observed. |
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Keywords: | biphenyl DDT DDT metabolites oxygen |
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