黄独脱毒苗叶片和茎段再生体系的建立

以黄独茎尖再生苗为试材，研究不同因素对黄独脱毒苗叶片和茎段再生体系的影响，以期对黄独脱毒苗的再生体系进行优化。结果表明，叶片和茎段诱导愈伤组织的最佳培养基是MS+KT 2 mg·L^-1+2,4-D 2 mg·L^-1；叶片和茎段诱导愈伤组织的最佳蔗糖浓度分别为30和50 g·L^-1；叶片和茎段在黑暗中较容易诱导出愈伤组织；叶片和茎段愈伤组织分化的最佳培养基是MS+KT 4 mg·L^-1+NAA 0.1 mg·L^-1；继代2次的叶片和茎段愈伤组织较容易分化；黄独不定芽生根的最佳培养基是1/2MS+IBA 0.1 mg·L^-1+NAA 0.5 mg·L^-1+PP₃₃₃ 1 mg·L^-1。本实验成功建立了黄独脱毒苗叶片和茎段的再生体系，为黄独脱毒苗的工厂化生产奠定了技术基础。

Establishment of Regeneration System of Leaves and Stems of Dioscorea bulbifera L. Virus-free Plantlets

Effects of different factors on regeneration of leaves and stems were studied by using Dioscorea bulbifera L. as test material to optimize the regeneration system of leaves and stems of D. bulbifera L. virus-free plantlets. The results showed: The callus induction medium of D. bulbifera L. leaves and stems was MS+KT 2 mg·L^-1+2,4-D 2 mg·L^-1; The best sucrose concentration of callus induction of D. bulbifera L. leaves and stems was 30 g·L^-1 and 50 g·L^-1 respectively; Calli of D. bulbifera L. leaves and stems were induced easily in dark; The differentiation medium of D. bulbifera L. leaf and stem calli was MS+KT 4 mg·L^-1+NAA 0.1 mg·L^-1; The calli of D. bulbifera L. leaves and stems were differentiated easily after subcultured for two times; The best rooting medium of D. bulbifera L. adventitious buds was 1/2MS+IBA 0.1 mg·L^-1+NAA 0.5 mg·L^-1+PP₃₃₃ 1 mg·L^-1. The regeneration system from leaves and stems of D. bulbifera L. virus-free plantlets was established successfully for the first time, which providing a technological basis for factory production of D. bulbifera L. virus-free plantlets.