Isolation and characterization of a <Emphasis Type="Italic">Bacillus</Emphasis> species capable of producing tetrodotoxin from the puffer fish <Emphasis Type="Italic">Fugu obscurus</Emphasis> |
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Authors: | Jun Wang Yanhui Fan |
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Institution: | (1) Department of Life Sciences, Binzhou University, 391, Huanghe Road 5, 256600 Binzhou, People’s Republic of China;(2) Shandong Key Laboratory of Eco-Environmental Science for Yellow River Delta, 256600 Binzhou, People’s Republic of China |
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Abstract: | We obtained puffer fish Fugu obscurus from Wudi, China and analyzed the level of tetrodotoxin (TTX) toxictiy by mouse bioassay. The ovary showed the highest potency
(125 MU/g), followed by the liver, intestine, and skin. A TTX-producing strain, namely, W-3, was isolated from the ovary of
puffer fish F. obscurus. After culturing at 28 °C for 48 h, toxins were extracted from the liquid medium and analyzed by mouse bioassay, high-performance
liquid chromatography, and gas chromatography-mass spectrometry. The results showed that strain W-3 produced TTX and related
compounds. Physiological and biochemical characterization and 16S rRNA analysis indicated that this strain represents a novel
species within the Bacillus genus; we named this strain as Bacillus sp. W-3. Our results suggested that marine bacteria play a role in the production of TTX in puffer fish F. obscurus. |
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