Comparison of Two PCR Systems for the Rapid Detection of <Emphasis Type="Italic">Leptospira</Emphasis> spp. from Kidney Tissue |
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| Authors: | Anne Mayer-Scholl Angelika Draeger Enno Luge Rainer Ulrich Karsten Nöckler |
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| Institution: | (1) Federal Institute for Risk Assessment (BfR), Berlin, Germany;(2) Friedrich-Loeffler-Institut (FLI), Institute for Novel and Emerging Infectious Diseases, Greifswald, Germany |
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| Abstract: | In this study we compared two routine PCR systems for the detection of Leptospira spp. and assessed their performance when
directly applied to kidney samples from small mammals. Although the kappa value of 0.9 indicated a high level of agreement
between the tests, the outer membrane lipoprotein gene lipl32 based PCR was more robust and showed a higher number of positive
kidney samples. |
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