The Distribution in Different Strains and Characteristic Features of Two Subfamilies of Drosophila melanogaster Retrotransposon MDG4 (gypsy) |
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| Authors: | Razorenova O V Karpova N N Smirnova Yu B Kousoulidou L K Renyova N K Subotcheva E A Kim A I Lyubomirskaya N V Ilyin Yu V |
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| Institution: | (1) Department of Genetics and Breeding, Moscow State University, Moscow, 119899, Russia;(2) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984, Russia |
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| Abstract: | The distribution of two variants of MDG4 (gypsy) was analyzed in severalDrosophila melanogasterstrains. Southern blot hybridization revealed the inactive variant of MDG4 in all strains examined and active MDG4 only in some of them. Most of the strains harboring the active MDG4 variant were recently isolated from natural populations. It is of interest that the active MDG4 prevailed over the inactive one only in strains carrying the mutantflamenco gene. Several lines were analyzed in more detail. The number of MDG4 sites on salivary-gland polytene chromosomes was established via in situ hybridization, and MDG4 was tested for transposition using the ovoD test. |
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