Relations between vitamin D and fatty acid binding properties of vitamin D-binding protein |
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Authors: | M Calvo J M Ena |
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Affiliation: | Technología y Bioquímica de los Alimentos, Facultad de Veterinaria de Zaragoza, Spain. |
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Abstract: | The interaction of fatty acids with bovine vitamin D-binding protein (DBP) was studied using a partition equilibrium method. This protein has one high affinity site for binding of fatty acids with an association constant Ka = 7 x 10(5) M-1 for palmitic acid and Ka = 6 x 10(5) M-1 for arachidonic acid. Competition experiments showed that palmitic acid hardly competes with 25-hydroxycholecalciferol for binding to DBP. However, arachidonic acid showed comparatively a stronger competition for binding to this protein. The great difference in competition of palmitic and arachidonic acids with 25-hydroxycholecalciferol may be related to changes in DBP conformation promoted by the binding of different ligands. |
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