High-efficiency plant regeneration from leaf explants of male himalayan poplar (Populus ciliata wall.) |
| |
Authors: | A. K. Thakur D. K. Srivastava |
| |
Affiliation: | (1) Department of Biotechnology, Dr Y. S. Parmar University of Horticulture and Forestry, Nauni, 173 230 Solan, HP, India |
| |
Abstract: | Summary This study reports a protocol for high-efficiency plant regeneration from leaf explants of male Himalayan poplar (Populus ciliata Wall.). Shoots were regenerated at high frequencies from explants grown on Murashige and Skoog (MS) medium supplemented with 0.5 mg l−1 kinetin and 0.2 mg l−1 indole-3-acetic acid (IAA). Regenerated shoots developed roots in MS medium supplemented with 0.1 mg l−1 IAA. Himalayan poplar plantlets could be produced within 2 mo. after acclimatization in a sterile mixture of sand and soil. |
| |
Keywords: | acclimatization direct organogenesis leaf explant Populus ciliata shoot regeneration |
本文献已被 SpringerLink 等数据库收录! |