Uracil phosphoribosyl transferase activity of mycoplasma and infected cell cultures |
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Authors: | C W Long R DelGiudice R S Gardella M Hatanaka |
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Institution: | (1) Viral Oncology Program, NCI Frederick Cancer Research Center, P.O. Box B, 21701 Frederick, Maryland;(2) Present address: Laboratory of DNA Tumor viruses, National Cancer Institute, National Institutes of Health, 20014 Bethesda, Md |
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Abstract: | Summary Human H. Ep-2 and mouse 3T6 cells infected byMycoplasma hyorhinis showed an increase in 3H]uracil uptake and a more than 20-fold increase in the activity of uracil phosphoribosyltransferase (UraPRT). Uninfected
cell cultures gave background levels of this enzyme activity. A survey of 16 strains of mycoplasma showed 13 to possess UraPRT
activity. Rabbit kidney cells (RK13) were infected with eight different strains of four mycoplasma species known to be common
cell culture contaminants. Seven of the eight cell cultures showed elevated UraPRT activities four days after infection. This
enzyme activity may be of value in monitoring cell cultures for mycoplasma and aid in classification.
This work was supported by Contract NO1-CP-53530 with the National Cancer Institute, National Institutes of Health, and Contract
FDA 74-41 of the Food and Drug Administration, Bethesda, Maryland 20014. |
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Keywords: | mycoplasma uracil phosphoribosyltransferase detection contaminated cell cultures |
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