Mounting Frozen Sections with Gelatin |
| |
Authors: | Mildred H Albrecht |
| |
Institution: |
a Department of Neurophysiology, Army Medical Service Graduate School, Washington, DC |
| |
Abstract: | Frozen sections, 15-50 µ thick, are soaked for 5 minutes or longer in a mixture of equal parts of 1.5% aqueous gelatin and 80% alcohol, and teased onto a slide. After allowing excess fluid to evaporate, sections will be moist and can be blotted with filter paper that may require dampening with 95% alcohol. Immersed in 95% alcohol, the remaining gelatin will congeal, anchoring the section to the slide. If necessary, the sections can subsequently be coated with celloidin. |
| |
Keywords: | |
本文献已被 InformaWorld 等数据库收录! |
|