Binding shift assay of parvalbumin, calmodulin and carbonic anhydrase by high-performance capillary electrophoresis |
| |
Authors: | H Kajiwara H Hirano K Oono |
| |
Institution: | National Institute of Agrobiological Resources, Ibaraki, Japan. |
| |
Abstract: | Shifts in mobility caused by binding of Ca2+ to calmodulin and parvalbumin were studied using high-performance capillary electrophoresis in a Tris-glycine buffer, rather than conventional polyacrylamide gel electrophoresis which requires larger amounts of sample and longer assay time. A Zn(2+)-binding protein, carbonic anhydrase, also showed a partial shift in mobility following Zn(2+)-binding. |
| |
Keywords: | |
|
|