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Calorie restriction alters mitochondrial protein acetylation |
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| Authors: | Bjoern Schwer Mark Eckersdorff Yu Li Jeffrey C. Silva Damian Fermin Martin V. Kurtev Cosmas Giallourakis Michael J. Comb Frederick W. Alt David B. Lombard |
| Affiliation: | Program in Cellular and Molecular Medicine, Howard Hughes Medical Institute, The Children's Hospital, and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA; Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109, USA; Cell Signaling Technology, Danvers, MA 01923, USA; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Abstract: | Calorie restriction (CR) increases lifespan in organisms ranging from budding yeast through mammals. Mitochondrial adaptation represents a key component of the response to CR. Molecular mechanisms underlying this adaptation are largely unknown. Here we show that lysine acetylation of mitochondrial proteins is altered during CR in a tissue-specific fashion. Via large-scale mass spectrometry screening, we identify 72 candidate proteins involved in a variety of metabolic pathways with altered acetylation during CR. Mitochondrial acetylation changes may play an important role in the pro-longevity CR response. |
| Keywords: | calorie restriction longevity mass spectrometry metabolism mitochondria sirtuins |