Solubilization of a high affinity CA-ATPase from dog antrum smooth muscle |
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Authors: | A K Grover P J Oakes S Samson C J Berenski C Y Jung |
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Institution: | McMaster University 1200 Main Street West Hamilton, Ontario, Canada, L8N 3Z5;Biophysics Laboratory Veterans Administration Medical Center State University of New York at Buffalo Buffalo, New York, U.S.A., 14215 |
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Abstract: | A Mg-independent high affinity Ca-ATPase has recently been reported to be present in the plasma membranes of smooth muscle. This enzyme has now been solubilized using deoxycholate. The membrane-bound and the solubilized enzymes resemble each other in Km for Ca2+, and inhibition by fluphenazine. The solubilized enzyme is, however, more sensitive to inhibition by Mg2+ than the membrane bound enzyme. Radiation inactivation analysis shows that whereas the membrane-bound enzyme had a target size of 98,000 +/- 4,000 Daltons, the solubilized enzyme was only 70,000, +/- 7,000 Daltons. |
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