The carbon monoxide Bohr effect in hemoglobin from Thunnus thynnus |
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| Authors: | M Brunori |
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| Affiliation: | 1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, PR China;2. Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian 116600, PR China;3. College of Pharmacy, Dalian Medical University, Dalian 116044, PR China;4. School of Chemical Engineering, Dalian University of Technology, Panjin 124221, PR China;1. Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan;2. Center for Clinical Pharmaceutical Sciences, School of Pharmacy, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan;3. School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia;4. Department of Pharmacokinetics and Biopharmaceutics, Institute of Biomedical Sciences, Tokushima University, 1-78-1, Sho-machi, Tokushima 770-8505, Japan;5. Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan;6. Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan;7. Faculty of Pharmaceutical Sciences and DDS Research Institute, Sojo University, 1-22-4 Ikeda, Nishi-ku, Kumamoto 860-0082, Japan |
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| Abstract: | The carbon monoxide Bohr effect of Thunnus thynnus hemoglobin was studied by the method of differential titrations. The results can be fitted by the simple model usually applied to mammalian hemoglobins, which assumes the presence of only two ligand-linked acid groups, one for the acid and one for the alkaline part of the effect. The values of ΔpK are considerably larger than those usually found in mammalian hemoglobins, being equal to 2.20 for the alkaline group and 1.20 for the acid group. The mean pK values for the two groups are consistent with the view that the two linked acid groups are a carboxyl in the acid range and an imidazole or an α-amino in the alkaline region. |
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