| Abstract: | Treatment of Chinese hamster ovary cells with 6-thioguanine (TG) produces severely disrupted chromatin in G2 cells, as visualized with premature chromosome condensation. In many regions of the chromosome this damage, appearing as a gapped or diffusely staining region, occurs in only one of sister chromatids. This morphology strongly supports previous reports that TG-induced cytotoxicity is related to its incorporation into DNA and that this lesion is expressed in the cell cycle following that in which drug exposure occurs. |
