黑叶猴和灰叶猴的线粒体DNA限制性片段长度多态研究

本文以15种限制性内切酶分析黑叶猴和灰叶猴种内及种间mtDNA多态。从各个样品中分别检出了41—50个酶切位点。综合15种限制内酶的酶切类型,在2只黑叶猴和2只灰叶猴中分别检出了两种限制性类型,并与其4个地理来源相对应。结合恒河猴和红面猴的资料,构建了4种猴科动物的分子系统树。结果表明,黑叶猴和灰叶猴种内的分歧分别始于30和35万年以前,两种叶猴的分离始于190万年以前,猴亚科和疣猴亚科的分离应早于1100万年。叶猴属在中国的扩散不是很晚才发生的。

MITOCHONDRIAL DNA RESTRICTION FRAGMENTLENGTH POLYMORPHISM IN Presbytis phayreiAND P.francoisi

Mitochondrial DNAs purified from the livers of P. phayrei and P. francoisi were analyzed with 15 restriction endonucleases. From the 15 6-bp recognition enzymes used, 41-50 sites were observed in the samples. By combining the cleavage patterns for each enzyme, the four samples were classified into four restriction types. The estimated number of nucleotide substitutions per site in P. phayrei, P. francoisi and between the two species was 0.007, 0.006 and 0.038, respectively. In combination with our previous data on Macaca mulatta and M. arctoides, the molecular phylogenetic trees of these four species were constructed by the unweighted-pair-group method and the neighbor-joining method. These results provide evidence that intraspecific divergence of P. phayrei and P. francoisi began about 0.3 Mybp, and that interspecific divergence between these two species and between Colobinae and Cercopithecinae began about 1.9 Mybp and before 11 Mybp, respectively.