Electrophoretic karyotypes of Phaffia rhodozyma strains |
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| Authors: | Ágnes Nagy Nándor Garamszegi Csaba Vágvölgyi Lajos Ferenczy |
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| Institution: | Chemical Works of Gedeon Richter Ltd., P.O. Box 27, H-1475 Budapest 10, Hungary; Department of Microbiology, Attila József University, P.O. Box 533, H-6701 Szeged, Hungary |
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| Abstract: | Abstract Electrophoretic karyotypes of strains from the astaxanthin-producing yeast Phaffia rhodozyma have been established. Intact chromosomal DNA molecules released from protoplasts were separated by orthogonal field alternation gel electrophoresis (OFAGE) and contour clamped homogeneous electric field (CHEF). Both small and large chromosomal DNA molecules were resolved simultaneously by optimizing the running conditions. Electrophoretic karyotypes among the Phaffia isolates examined differed significantly. Seven to thirteen chromosomal bands, ranging in size from 0.83 Mb to 3.50 Mb, were resolved, giving total genome sizes of about 15.4 to 23.2 Mb. Ribosomal DNA has been assigned to chromosomal bands using a heterologous gene probe. |
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| Keywords: | Phaffia rhodozyma Orthogonal field alternation gel electrophoresis Clamped homogeneous electric field Electrophoretic karyotype Gene localization |
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