Analysis of proximal X chromosome pairing in early female mouse meiosis |
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Authors: | Christine O’Keeffe Maj A Hultén Charles Tease |
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Institution: | (1) Mammalian Genetics Unit, Medical Research Council, Harwell, Didcot, Oxon OX11 0RD, UK, ML;(2) LSF Research Unit, Regional Genetics Services, Birmingham Heartlands Hospital, Birmingham B9 5PX, UK, GB |
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Abstract: | The initiation and progression of homologous chromosome pairing at meiosis were investigated in female mice. The proximal
end of the X chromosome was identified in fetal oocytes using fluorescence in situ hybridisation with the repeat copy probe
70-38. The X centromeres appeared to be randomly positioned in the nuclei from pre-meiotic interphase to leptotene. The observations
indicated no pre-synaptic association for the proximal end of the X chromosome. There was a significant increase in the number
of paired X centromeres from mid-zygotene to late zygotene. The proximal end of the X chromosome is therefore a generally
late pairing region with no significant association seen before mid-zygotene. The centromeric heterochromatin of all chromosomes
could be seen to associate into varying numbers of clusters during pre-leptotene through to pachytene. These clusters do not
seem to be directly involved in bringing homologues together, as X centromeres did not consistently localise to the same cluster.
Received: 1 August 1996; in revised form: 1 June 1997 / Accepted: 1 June 1997 |
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