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A high-performance liquid chromatography method for the assay of cytidine monophosphate-sialic acid synthetase |
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| Authors: | C R Petrie W Korytnyk |
| Affiliation: | 1. Department of Experimental Therapeutics, Roswell Park Memorial Institute, New York State Department of Health, 666 Elm Street, Buffalo, New York 14263 USA;2. Grace Cancer Drug Center, Roswell Park Memorial Institute, New York State Department of Health, 666 Elm Street, Buffalo, New York 14263 USA |
| Abstract: | An HPLC method has been developed for the assay of cytidine monophosphate-sialic acid synthetase (EC 2.7.7.43) using ion-pair chromatography and gradient elution. This procedure permits the assay of alternative substrates and inhibitors of the enzyme and is not subject to the limitation of the colorimetric method. The newly synthesized N-acetyl-9-deoxy-9-fluoro-D-neuraminic acid was found to be a good substrate of the enzyme with a Km of 6.35 mM as compared to 1.84 mM for N-acetylneuraminic acid. |
| Keywords: | To whom requests for reprints should be addressed. |
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