Mapping of quantitative trait loci and development of allele-specific markers for seed weight in Brassica napus |
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Authors: | Chuchuan Fan Guangqin Cai Jie Qin Qingyuan Li Minggui Yang Jianzhong Wu Tingdong Fu Kede Liu Yongming Zhou |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China; |
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Abstract: | Seed weight is an important component of grain yield in oilseed rape (Brassica napus L.), but the genetic basis for the important quantitative trait is still not clear. In order to identify the genes for seed
weight in oilseed rape, QTL mapping for thousand seed weight (TSW) was conducted with a doubled haploid (DH) population and
an F2 population. A complete linkage map of the DH population was constructed using 297 simple sequence repeat (SSR) markers. Among
nine TSW QTLs detected, two major QTLs, TSWA7a and TSWA7b, were stably identified across years and collectively explained 27.6–37.9% of the trait variation in the DH population. No
significant epistatic interactions for TSW detected in the DH population indicate that the seed weight variation may be primarily
attributed to additive effects. The stability and significance of TSWA7a and TSWA7b were further validated in the F2 population with different genetic backgrounds. By cloning BnMINI3a and BnTTG2a, two B. napus homologous genes to Arabidopsis thaliana, allele-specific markers were developed for TSWA5b and TSWA5c, two TSW QTLs on A5, respectively. The importance of the major and minor QTLs identified was further demonstrated by analysis
of the allelic effects on TSW in the DH population. |
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