Acquired resistance to the rice blast in transgenic rice accumulating the antimicrobial peptide thanatin |
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Authors: | Tomohiro Imamura Michiko Yasuda Hiroaki Kusano Hideo Nakashita Yuko Ohno Takashi Kamakura Seiichi Taguchi Hiroaki Shimada |
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Institution: | (1) Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan;(2) Research Institute of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan;(3) Plant Acquired Immunity Research Unit, Advanced Science Institute, RIKEN, Wako Saitama, 351-0198, Japan;(4) Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo 060-0810, Japan;(5) Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan;(6) Present address: Iwate Biotechnology Research Center, Kitakami 024-003, Japan; |
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Abstract: | Thanatin is an antimicrobial peptide with a strong and wide-ranging antimicrobial spectrum, including certain species of fungi
and Gram-negative and Gram-positive bacteria. To evaluate the application of thanatin to the generation of disease-resistant
plants, we introduced a synthetic thanatin gene into rice. Several transformants that expressed the introduced gene showed
significant level of antimicrobial activity. The substances showing antimicrobial activity were partially purified from these
transformants and their properties were determined. The molecule with characteristics similar to those of native thanatin
on the elution pattern in HPLC analysis had an identical molecular mass to that of native molecule. It should also be noted
that the transformant acquired a sufficient level of resistance to the rice blast fungus, Magnaporthe oryzae, presumably due to the repressive activity of thanatin to its initial stage of infection. This result demonstrates that thanatin
has antifungal activity for M. oryzae and that the introduction of the thanatin gene into rice is effective in generating a plant resistant to rice blast disease. |
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