Comparison of an oligo-chip based assay with PCR method to measure the prevalence of tick-borne pathogenic bacteria in central Slovakia |
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| Authors: | Elena Kocianová Du?an Bla?kovi? Katarína Smetanová Katarína Schwarzová Vojtech Boldi? Zina Ko??anová Denisa Müllerová Imrich Barák |
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| Institution: | (1) Institute of Virology, Slovak Academy of Science, Dúbravská cesta 9, SK-84505 Bratislava, Slovakia;(2) Institute of Molecular Biology, member of the Centre of Excellence for Molecular Medicine, Slovak Academy of Sciences, SK-84551 Bratislava 45, Slovakia;(3) Regional Office of Public Health in Žiar nad Hronom, Sládkovičova 484/9, SK-96524 Žiar nad Hronom, Slovakia |
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| Abstract: | Ticks are well-known vectors for a wide range of pathogenic microorganisms. We examined the presence of Rickettsia spp., Anaplasma spp., Borrelia spp., Coxiella burnetii and Francisella tularensis in Ixodes ricinus ticks collected in central Slovakia using oligo-chip based assay. Rickettsiae were detected in 5.6% of examined ticks. Borreliae
and anaplasmae were identified in 2.1% and 2.8% ticks, respectively. All tested samples were negative for presence of Coxiella burnetii and Francisella tularensis. All these results were compared with those obtained by PCR analysis, and a close correlation between them was found. In
addition, rickettsiae of spotted fever group (SFG), Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato were found in ticks using genera or species-specific PCR methods. They are circulating in 10 out of 18 studied
localities. |
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| Keywords: | tick-borne pathogenic bacteria Ixodes ricinus polymerase chain reaction DNA-chip |
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