A Novel Electrochemically Active and Fe(III)-reducing Bacterium Phylogenetically Related to Clostridium butyricum Isolated from a Microbial Fuel Cell |
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Authors: | Hyung Soo Park Byung Hong Kim Hyo Suk Kim Hyung Joo Kim Gwang Tae Kim Mia Kim In Seop Chang Yong Keun Park Hyo Ihl Chang |
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Institution: | a Water Environment Research Centre, Korea Institute of Science and Technology, Hawolgok-dong, Sungpook-ku, Seoul, 136-791, Korea;b Graduate School of Biotechnology, Korea University, Anam-dong, Sungpook-ku, Seoul, 136-701, Korea |
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Abstract: | An obligatory anaerobic bacterium was isolated from a mediator-less microbial fuel cell using starch processing wastewater as the fuel and designated as EG3. The isolate was Gram-positive, motile and rod (2.8–3.0 μm long, 0.5–0.6 μm wide). The partial 16S rRNA gene sequence and analysis of the cellular fatty acids profile suggested that EG3 clusters with Clostridium sub-phylum and exhibited the highest similarity (98%) with Clostridium butyricum. The temperature and pH optimum for growth were 37°C and 7.0, respectively. The major products of glucose and glucose/Fe(O)OH metabolism were lactate, formate, butyrate, acetate, CO2and H2. Growth was faster at the initial phase and the cell yield was higher when the medium was supplemented with Fe(O)OH than without Fe(O)OH. These results suggest that Fe(III) ion is utilised as an electron sink. Cyclic voltammetry showed that Clostridium butyricum EG3 cells were electrochemically active. It is a novel characteristic of strict anaerobic Gram-positive bacteria. |
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Keywords: | Clostridium sp EG3 dissimilatory Fe(III) reduction cyclic voltammogram electrochemically active bacteria microbial fuel cell |
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